Health Research Registry List

Research Registry No : HRID-00637_V1
 
University Departmental Research : Yes
 
Name of University : University of Medical Technology, Yangon
 
Department : Medical Laboratory Technology
 
Registration Date : 2020-03-14
 
Title of Research : Detection of Rh DEL phenotype by using adsorption elution method and its association with other Rh antigens among Rh negative blood donors
 
Principle Investigator : Saw Thu Wah
 
Co-authors : Daw Saung Nay Chi, Daw Kyi Kyi Kyaing, Prof. Dr Aye Aye Khin, Dr. Thida Aung
 
Field of Research : Clinical Haematology
 
Publication Source : Advances in Hematology. 2020;2020
 
 
Year of Publication : 2020
 
URL of Publication : -
 
Presentation Source :
 
Placement of Presentation : Hindawi
 
Year of Presentation : 2020
 
Abstract : Background. Red cell Rhesus (Rh) antigen expression is influenced by the genetic polymorphism of RHD and RHCE genes and reveals serologically different reactions of RhD variants such as partial D, weak D, and Rh-Del. Serologically, Rh-Del type can only be detected by an adsorption-elution technique, and it might be mistyped as Rh-negative. e prevalence of Rh-Del has not been reported yet in Myanmar. Method. A total of 222 Rh-negative blood donors in the National Blood Center were tested for weak D and Rh-Del by indirect anti-human globulin and adsorption-elution method, respectively. RhCE typing was performed among Rh-negative and Rh-Del. Results. Of them, 75.2% (167/222) were Rh-negative, 15.8% (35/222) were Rh-Del, and 9% (20/222) were weak D. Of 202 blood donors (167 true Rh-negative and 35 Rh-Del), all of the Rh-Del positives were C-antigen-positive with 94.3% Ccee phenotype (33/35) and 5.7% CCee (2/35). Most of the Rh-negative donors (80.2%) were ccee phenotype (134/167). Conclusion. About half of Rh-Del subjects were repeated donors, and attention was needed to avoid transfusion of truly Rh-negative patients to prevent alloimmunization. It is recommended to do Rh-Del typing of Rh-negative donors who are C-antigen positive and consider moving them to the Rh-positive pool. Further study is needed to clarify the alloimmunization status for transfusion of Rh-Del blood to Rh-negative recipients. Molecular markers for RhD-negative and D variants should be established in the Myanmar population to improve the selection of antisera for Rh typing and enhance the safety of the transfusion services.
 
 
IRB/PRC/ERC Approval Date : 2018-04-04
 
Placement of IRB/PRC/ERC : University of Medical Technology
 
IRB/PRC/ERC Approval Letter/Document : 14032020100759IRBcertificate(DEL).pdf
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Website : -