Preliminary Registration Detail

Research Registry No : PLRID-00736_V4
 
Postgraduate Research : No
 
University Departmental Research : Yes
 
Name of University : University of Medicine 2, Yangon
 
Department : Department of Biochemistry
 
Registration Date : 2020-03-25
 
Principle Investigator : Swe Mar Oo
 
Co-Investigators : May Pyone Kyaw, Ye Win Tun, Min Thar Htut, Aye Aye Mon
 
Title of Research : Effect of regular exercise on blood mitochondrial DNA copy number and its association with plasma Selenoprotein P levels in apparently healthy young adults from two institutions in Myanmar
 
Field of Research : Biochemistry
 
Preliminary Research Design : Experimental Study
 
Preliminary Research Type : Basic
 
Preliminary Justification : Mitochondria are the dynamic cellular organelles whose major function is the production of chemical energy from metabolic fuels. As they are the major site of energy production in the cell, the numbers and/or functions of mitochondria can be regarded as an important factor for total energy metabolism of the whole body, especially during active metabolic condition such as physical exercise. It was previously reported that Reactive oxygen species (ROS) signaling during acute exercise induces mitochondrial biogenesis to fulfil higher energy demand, whereas those changes during regular exercise on mitochondrial dynamic hasn’t yet been clearly reported. Selenoproteins P (SELENOP) is a major selenium transport protein with anti-oxidant action. Overproduction of SELENOP and its excessive removal of ROS was reported to be associated with insulin resistance and exercise resistance in skeletal muscle by interfering the ROS-mediated signaling pathways. However, it is still not clear that the suppressive effect of SELENOP on exercise effectiveness is mediated by suppression of mitochondrial biogenesis. To date, relationship between plasma SELENOP and mitochondrial dynamic hasn’t yet been clarified in human studies. On the other hand, while the reduction of plasma SELENOP is considered as the potential target for the prevention and treatment of metabolic diseases, exploration of various factors which can influence plasma SELENOP became the interest of many researchers. To our best knowledge, there has been very few and unclear reports concerning with the effect of regular exercise on the plasma levels of SELENOP especially in young healthy population. On this regards, the first aim of this study is to evaluate the effect of regular exercise training on mitochondrial dynamic by measuring the blood mitochondrial DNA copy number. Then, this study will attempt to analyze the effects of regular exercise on the concentrations of plasma SELENOP levels. Finally, the current study tends to evaluate the influence of plasma SELENOP levels on that exercise induced changes in mitochondrial dynamic. It will give better understanding of the role of cellular redox status, mitochondrial dynamic and plasma SELENOP levels in metabolic adaptations of physical exercise, which will be useful for the consideration of more effective intervention in the management of metabolic diseases.
 
Preliminary Aim : To study the effect of regular exercise on blood mitochondrial DNA copy number and its association with plasma selenoprotein P levels in apparently healthy young adults from two institutions in Myanmar
 
Preliminary Objectives : 1. To determine blood mitochondrial DNA copy number and plasma selenoprotein P levels in regular exercised and non-exercise subjects 2. To compare blood mitochondrial DNA copy number in non-exercised and regular exercised subjects 3. To compare plasma SELENOP concentrations in non-exercised and regular exercised subjects 4. To assess the association between plasma SELENOP levels and blood mitochondrial DNA copy number in study population
 
Preliminary Sample Size : 44 each of healthy volunteer students will be recruited from Institute of Sports and Physical Education (ISPE), Yangon and University of Medicine 2 Yangon, as regular exercised group and non-exercised group respectively
 
Preliminary Study Duration : One year (from December 2019 to December 2020)
 
Preliminary Study Area : 1. Institute of Sports and Physical Education (ISPE), Yangon 2. University of Medicine 2, Yangon
 
Preliminary Study Method : Blood mitochondrial DNA copy number and plasma selenoprotein P levels will be measured and compared between regular-exercised and non-exercised group. The association between the two variables will be tested in each group and total population.
 
Preliminary Research Outcome : The study will help for the better understanding of the role of physical exercise in the regulation of mitochondrial dynamic and plasma selenoprotein P levels, which will be a useful information for the clinician to consider more effective and comprehensi
 
Preliminary Research Finding : Sample collection and biochemical analysis will be performed in April and May, 2020.
 
Preliminary Progress Report : April, May 2020 - participant recruitment and sample collection May 2020 - laboratory procedure and data analysis
 
Study Starting Date : 2019-12-31
IRB/PRC/ERC Approval Date : 2020-03-24
 
Placement of IRB/PRC/ERC : 30-01-2020
 
IRB/PRC/ERC Approval Letter/Document : 250320200629172019-160Dr.SweMarOo.pdf
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Name of Organization Website : -