Preliminary Registration Detail

Research Registry No : PLRID-00288_V2
 
Postgraduate Research :
 
University Departmental Research : yes
 
Registration Date : 2019-04-01
 
Principle Investigator : Khin Aye Thin
 
Co-Investigators : Professor Dr. Aye Aye Khin, Professor/Head, Department of Medical Laboratory Technology, University , Win Pa Pa Soe, Laboratory Officer, Department of Food and Drug Administration, Yangon, Myanmar., Mi Hnin Htet Htet Oo, Demonstrator, Department of Medical Laboratory Technology, University of Medic
 
Title of Research : DETECTION OF AFLATOXIN M1 FROM MILK BY ELISA METHOD
 
Field of Research : Public Health
 
Preliminary Research Design : Descriptive Study
 
Preliminary Research Type : Basic
 
Preliminary Justification : Aspergilus species are widespread and can saprophyte on foods and feeds. During their life cycle, they can produce a wide range of mycotoxins including aflatoxins which are carcinogenic. Aflatoxins can be produced by A. flavus, A. parasiticus and A. nomius. Among many types of aflatoxins, aflatoxin B1 has teratogenic property and the greatest acute toxicity to animal and was recognized as the most carcinogenic type (group I carcinogen) (Barkai-Golan, 2008) (IARS, 2002). Aflatoxin B1 can be metabolized to the monohydroxy derivative aflatoxin M1 (AFM1) in liver of cows and secreted into the milk (Bilandzic et al., 2014). AFM1 is also acutely toxic, hepatotoxic as aflatoxin B1 and classified as group II carcinogen (International agency, 2002). It cannot be destroyed during pasteurization and even with the high temperature (Badea et al., 2004) (Iha et al., 2013). It can contaminate milk and milk products which are potential source of nutrients. Therefore, some countries have laid down maximum residue limit (MRL) of AFM1. MRL of AFM1 in milk and milk products for European Community is 0.05 μg/L (European commission, 2006) and that of USA and China is less than 0.5 μg/L (US food and drug administration, 2005) (MoH P.R. China, 2011). However, many countries in the world have not set the limit of AFM1 in milk (Rastogi, 2003). The documented minimum and maximum occurrences of AFM1 in milk are : • 0.002 and 0.083 μg/L for pasteurized milk (Rastogi et al., 2004) in Taiwan, • 0.006 and 0.528 μg/L for pasteurized milk (Fallah, 2010), 0.015 and 0.28 μg/L for raw milk (Kamkar, 2006) in Iran, • 0.001 and 0.117 f μg/L or pasteurized milk (Zinedine et al., 2007) in Morocco, • 0.002 and 0.08 μg/L for raw milk (Lee et al., 2009) in South Korea, • 0.05 and 0.101 μg/L for raw milk (Ruangwises, 2010) in Thailand, • 0.22 and 6.9 μg/L for raw milk (Elzupir, 2010) in Sudan, • 0.0033 and 0.084 μg/L for pasteurized milk, 0.0026 and 0.126 μg/L for raw milk (Assem, 2011) in Lebanon, • 0.009 and 0.437 μg/L for pasteurized milk (Iha et al., 2013) in Brazil, • 0.16 and 0.5 μg/L for raw milk (Pei et al., 2009), 0.005 and 0.06 μg/L for raw milk (Han et al, 2013) in China, • 0.004 and 0.845 μg/L for raw milk (Iqbal, 2013) in Pakistan, • 0.006 and 0.027 μg/L for raw milk (Bilandzic et al., 2014) in Croatia, • 0.6 and 1.2 μg/L for pasteurized milk (Kos et al., 2014), 0.08 and 1.2 μg/L for raw milk (Tomasevic et al, 2015) in Serbia. This study will find out the contamination level of AFM1 from locally available milk, pasteurised milk, ultra heat treated milk and as raw milk.
 
Preliminary Aim : To detect Aflatoxin M1 from locally available milk
 
Preliminary Objectives : (1) To determine the contamination level of Aflatoxin M1 from raw milk by ELISA method (2) To determine the contamination level of Aflatoxin M1 from pasteurized milk by ELISA method (3) To determine the contamination level of Aflatoxin M1 from ultra-heat
 
Preliminary Sample Size : Thirty milk samples containing ultra-heat treated milk, pasteurized milk and raw milk
 
Preliminary Study Duration : one year
 
Preliminary Study Area : Yangon, Myanmar
 
Preliminary Study Method : convenient sampling method
 
Preliminary Research Outcome : It is hoped that this study can find out health hazard from milk and support the prevention of diseases. And, it may contribute facts for further researches and guidelines.
 
Preliminary Research Finding : AFM1 levels in samples are determined from the standard curve which showed excellent linearity r2 value of 0.99. The coefficient of variation of each standard absorbance value was less than 10 %. The detection limit of ELISA in this study was 0.005 μg/L.
 
Preliminary Progress Report : Twenty five samples of pasteurized milk and 5 samples of raw milk have been collected and performed for detection of AFM1.
 
Study Starting Date : 2018-08-01
IRB/PRC/ERC Approval Date : 2018-04-04
 
Placement of IRB/PRC/ERC : University of Medical Technology, Yangon
 
IRB/PRC/ERC Approval Letter/Document : Ethical document AFM1.pdf
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Name of Organization Website : -